Sequence-dependent cytotoxicity of second-generation oligonucleotides
نویسندگان
چکیده
منابع مشابه
Sequence-dependent cytotoxicity of second-generation oligonucleotides.
In this study, we have examined the potential of second-generation antisense chimeric 2'-O-(2-methoxy)ethyl/DNA phosphorothioate oligonucleotides (ONs) to affect cell growth through non-antisense mechanisms. Evaluation of a series of ONs demonstrated that only a small number were cytotoxic at concentrations close to those required for antisense activity. Toxicity of the ONs appeared to be seque...
متن کاملSequence Dependent Mobility of Short Oligonucleotides in Free- Solution Electrophoresis
INTRODUCTION Since completion of the human genome project, many additional organisms have been sequenced and ~1.8 million human genetic Single Nucleotide Polymorphisms (SNPs) have been identified and documented by the SNP consortium, http://snp.cshl.org. While the technology workhorses for these efforts have been gel-based and capillary electrophoresis DNA sequencers, there is increased interes...
متن کاملSecond-generation antisense oligonucleotides: structure-activity relationships and the design of improved signal-transduction inhibitors.
630 Second-generation antisense oligonucleotides: structure-activity relationships and the design of improved signal-transduction inhibitors K.-H. Altmann*, D. Fabbrot, N. M. Dean$, T. Geigert, 6. P. Mania$, M. Mullert and P. Nickling *Ciba-Geigy Ltd., Central Research Laboratories, RI060 2.34, CH-4002 Basel, Switzerland, tCiba-Geigy Ltd , Pharmaceutical Division, Department of Oncology, CH-400...
متن کاملSynthesis, biophysical properties and biological activity of second generation antisense oligonucleotides containing chiral phosphorothioate linkages
Bicyclic oxazaphospholidine monomers were used to prepare a series of phosphorothioate (PS)-modified gapmer antisense oligonucleotides (ASOs) with control of the chirality of each of the PS linkages within the 10-base gap. The stereoselectivity was determined to be 98% for each coupling. The objective of this work was to study how PS chirality influences biophysical and biological properties of...
متن کاملIn vivo generation of highly abundant sequence-specific oligonucleotides for antisense and triplex gene regulation.
Antisense and triplex oligonucleotides continue to demonstrate potential as mediators of gene-specific repression of protein synthesis. However, inefficient and heterogeneous cellular uptake, intracellular sequestration, and rapid intracellular and extracellular degradation represent obstacles to their eventual clinical utility. Efficient cellular delivery of targeted ribozymes can present simi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2004
ISSN: 1362-4962
DOI: 10.1093/nar/gkh997